Horizontal transfer of antibiotic resistance genes into microflora and blood cells in rats fed on GM-diet

Abstract Background With regard to the extensive production of genetically modified organisms, scientists focused on safety antibiotic resistance marker (ARM) genes present in GM-crops produced for consumption human or animal. We investigated possible transfer these microflora gastrointestinal tract and blood cells two groups Male Wistar Albino rats fed a transgenic diet containing ARM nptII aadA 90 days. The conventional PCR was employed screen presence experimental diets, animals’ samples. Results occurrence DNA from GM-diet bacterial has been unambiguously demonstrated. were further confirmed by sequencing blast analysis, indicating that both transferred segments shared significant alignment with number Binary Cloning Vectors variable similarities. frequency into unexpectedly higher than its which may be attributed factors. Conclusions Our results demonstrated ( aadA) GM plant enteric rats. draw attentions importance exploring effects horizontally products consumers extend our better understanding factors influencing HGT intestine GM-food consumers.